نهمین کنفرانس ملی و اولین کنفرانس بین المللی فیزیولوژی گیاهی

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کد مقاله 1023

عنوان Identification of Key Transcription Factors in Cucumber (Cucumis sativus): Linking the Circadian Clock with Heat Stress Response

نویسندگان Zahra Zinati - Farideh Farahbakhsh - Zahra Amjadi

چکیده Climate change-induced heat stress is a major threat to cucumber (Cucumis sativus) productivity, impairing growth, photosynthesis, and yield. To identify key transcriptional regulators of the heat stress response, we conducted an RNA-seq analysis on leaves of cucumber seedlings exposed to 42°C at 3- and 6-hour post-treatment (accession PRJNA634519). Using stringent criteria (FDR < 0.00001, |log2FC| ≥ 4), we identified 166 significantly differentially expressed genes (DEGs). Among these, eight were identified as transcription factors (TFs), including members of the HSF, MYB, WRKY, NAC, Trihelix, GARP, and B3 families. Notably, several of these TFs, such as REVEILLE 6 (RVE6), REVEILLE 8 (RVE8), and PCL1, are known regulators of the circadian clock, suggesting a close link between circadian rhythms and heat stress response in cucumber. Additionally, up-regulation of NAC, WRKY, and HSF TFs underscores their established roles in abiotic stress defense. The significant downregulation of a B3 domain-containing TF implies it may act as a repressor relieved under stress. Our findings highlight a complex regulatory network where circadian-associated TFs potentially coordinate the timely activation of heat-responsive genes. These key TFs provide valuable targets for future functional validation and breeding strategies to enhance thermotolerance in cucumber.

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