نهمین کنفرانس ملی و اولین کنفرانس بین المللی فیزیولوژی گیاهی

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کد مقاله 1149

عنوان Effect of biotic and abiotic elicitors on Teucrium polium L. callus in vitro

نویسندگان Fahimeh Gharaei - Hadiseh Sadat Hosseini Pouya - Monireh Cheniany

چکیده Plant tissue culture is a modern biotechnology technique that enables the detailed study of physiological processes and the regulation of cell growth under controlled condition. In this study, the effects of two types of elicitors, biotic (chitosan) and abiotic (silver nitrate), on induction and growth parameters of callus of the medicinal plant Teucrium polium L. were investigated. Leaf explants were cultured on MS medium supplemented with various concentrations of the hormones BAP and NAA, followed by treatments with different elicitors concentrations. The results showed that the main effects of hormones and elicitors, and their interactions, on the fresh and dry weight of callus were significant (p ≤ 0.01). The highest fresh weight of callus was observed in the combined treatment of BAP (1.5 mg L-1) + NAA (0.5 mg L-1) with chitosan at 150 mg L-1. In addition, silver nitrate at 12 mg L-1 also positively affected callus growth. Findings suggest that the application of biotic elicitor chitosan and abiotic elicitor silver nitrate can improve cell growth and division in T. polium tissue culture by stimulating physiological pathways and hormonal regulation.

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