نهمین کنفرانس ملی و اولین کنفرانس بین المللی فیزیولوژی گیاهی

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کد مقاله 1148

عنوان Utilizing evolutionary and structural evidence to identify key regulatory elements in genistein biosynthesis in Medicago truncatula

نویسندگان Hadiseh Sadat Hosseini Pouya - Parviz Heidari - Monireh Cheniany

چکیده Genistein is an important isoflavone in legumes (including the model species Medicago truncatula). This metabolite has notable medicinal and agricultural importance. The present study aims to identify key evolutionary and structural features and to analyze the cis-regulatory elements of the HID and IFS gene families, which encode two essential enzymes in the genistein biosynthesis pathway. For this purpose, in silico analyses were performed using M. truncatula genome, and the evolutionary relationships and protein structures of HID genes were also examined. In addition, promoter sequences of all MtHID and MtIFS genes were analyzed. The results showed that the HID protein structure is conserved across legumes, and promoter analysis revealed that stress-responsive elements, particularly MYB and MYC, are the most abundant regulatory motifs in both gene families. Additionally, ABA-responsive elements were the most abundant in MtHID promoters, while both ABA- and MeJA-responsive elements were dominant in MtIFS promoters. Based on these results, the cis elements suggest a multi-layered regulatory structure in the genistein biosynthesis pathway. These findings provide valuable insights for future approaches to enhance genistein production through targeted manipulation of regulatory networks and metabolic engineering.

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